Opening Black Box of Soil Microbial Diversity through Molecular Techniques

http://www.biotecharticles.com/Biotech-Research-Article/Opening-Black-Box-of-Soil-Microbial-Diversity-through-Molecular-Techniques-3777.html

The vast majority of microbes have not yet been isolated, identified or characterized. The advent of culture-independent techniques has transformed the field of microbiology and soil microbial ecology in particular. PCR-based techniques such as DGGE, TGGE, T-RFLP, allow the classification of microorganisms based on particular genetic markers such as 16S rRNA and the profiling of complex microbial communities on the basis of sequence diversity.